How scientists decipher COVID-19 —Part 2: Sanger and Next Generation Sequencing

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Introduction

How NGS works

Why NGS is more preferable than Sanger?

1. Capture a broader spectrum of mutations.

2. Genomes are interrogated objectively.

3. Allow detection of mosaic mutations.

4. Determine the identity of pathogens through genomic data (microbiology)

5. Advance cancer genome research (oncology)

Limitations

Economic limitation

Technical limitation

References

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